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. 2018 Dec 29;24(1):110. doi: 10.3390/molecules24010110

Figure 5.

Figure 5

Akt phosphorylation is involved in SHK-mediated protective effects. (A) Protein and small molecule/chemical interaction analysis of SHK using STITCH (version 5.0). (B,C) L-02 cells were incubated with SHK for the indicated durations, and then the levels of Akt phosphorylation were evaluated by Western blotting. (D) L-02 cells were pretreated with LY294002 (20 μM) for 1 h, followed by treatment with SHK and LY294002 for 8 h, and then the Nrf2 protein levels were evaluated by Western blotting. (E,F) Cells were pretreated with LY294002 (20 μM) for 1 h, followed by treatment with SHK and LY294002 for 1 h, and then the cells were exposed to APAP for 24 h, then cell viability and GSH depletion was assessed. All of the data shown represent the average from three independent experiments. * p < 0.05 versus the control group; # p < 0.05 versus the APAP group; ^ p < 0.05 versus the SHK plus APAP group.