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. 2018 Dec 29;24(1):110. doi: 10.3390/molecules24010110

Figure 6.

Figure 6

Effect of SHK on Akt-induced GSK3β phosphorylation and Nrf2 upregulation. (A,B) L-02 cells were incubated with SHK for the indicated durations, and then the levels of GSK3β phosphorylation were evaluated by Western blotting. (C) L-02 cells were pretreated with LY294002 (20 μM) for 1 h, followed by treatment with SHK for 4 h, and GSK3β protein and phosphorylated GSK3β levels were evaluated by Western blotting. (D) L-02 cells were pretreated with lithium chloride (20 mM) for 4 h, followed by treatment with SHK for 4 h, and GSK3β protein and phosphorylated GSK3β levels were evaluated by western blotting.