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Schematic illustration of cell engineering using copper-free click chemistry. (A) Azide-modified cells are labeled with DBCO-imageable probes via SPAAC reaction. The modified cells are detected after transplantation; (B) Azide-modified MSCs are labeled with DBCO-coated nanoparticles via SPAAC reaction. The modified MSCs home to tumor tissues after transplantation; (C) Tz-modified cells and TCO-modified cells form two- or multiple-cell pairs via iEDDA reaction.
