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. 2018 Dec 25;20(1):77. doi: 10.3390/ijms20010077

Figure 1.

Figure 1

CCL2 expression is involved in ICAM1 upregulation in AF cells. Stimulation with LPS plus IFNγ increased total and cell surface expression of ICAM1 as determined by western blotting (A), and flow cytometry (C). (B) AF cells were stimulated with LPS plus IFNγ for indicated times, and the mRNA level of ICAM1 was determined by real-time PCR. (D) Following stimulation of AF cells with LPS plus IFNγ, the mRNA levels of CCL2 and CCL3 were determined by real-time PCR. (E) After incubation with CCL2-neutralizing antibody or control IgG followed by stimulation with LPS plus IFNγ, ICAM1 cell surface expression was determined by flow cytometry. Note that CCL2 expression was increased in stimulated AF cells, and that CCL2 was an endogenous regulatory mediator in ICAM1 expression. The results are expressed as the mean ± standard error of the mean from three independent experiments. * p < 0.05 compared with the control group. # p < 0.05 compared with the LPS/ IFNγ treatment group.