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. 2018 Dec 21;20(1):21. doi: 10.3390/ijms20010021

Figure 3.

Figure 3

5-aza treatment exerts sustained apoptotic impact on EC cell lines. (a) PARP1 cleavage remains high in NCCIT and NCCIT-R cells, while 2102EP and 2102EP-R cells seem to lose PARP1 degradation over time. (b) Caspase-3 cleavage continues in all 4 cell lines, but to a lesser extent in both cisplatin-resistant isogenic sublines. Interestingly, after a 168 h-break after 5-aza exposure, 5µM cisplatin seemed to induce delayed apoptosis to the highest degree. (c) Schematic experimental design of cell cycle analyses (TP, time point). (d) Flow cytometric analysis of PI-positive apoptotic cells among all tested cell lines. (e) Evaluation of the experiments conducted in D. Even a week after the end of 5-aza treatment, TP53-mutant NCCIT and NCCIT-R cells underwent apoptosis more frequently when compared to untreated control cells. Shown are mean ± standard deviation (SD) of experiments, which were repeated three times with similar results.