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. 2019 Jan 8;20(1):225. doi: 10.3390/ijms20010225

Figure 1.

Figure 1

Methylseleninic acid (MSA) is toxic to Cal27 and SCC25 HNSCC cells in a dose- and time-dependent manner. (A) PI exclusion assay of Cal27 cells treated with the shown concentrations of Se-methylselenocysteine (MSC), seleno-l-methionine (SLM), or MSA for 72 h. (B) Propidium iodide (PI) exclusion assay of SCC25 cells treated with 0 to 10 µM MSA for 72 h. (C) Cell counts of Cal27 and SCC25 cells following treatment with 10 µM MSA for 0 to 72 h. (D) PI exclusion assay of Cal27 and SCC25 cells after treatment with 10 µM MSA for 0 to 72 h. *, statistical significance relative to 0 µM MSA controls; p < 0.05, n = 3.

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