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. 2019 Jan 4;20(1):161. doi: 10.3390/ijms20010161

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE analysis (non-reducing conditions) of the chromatographic fractions generated by purifying Mab from a CHO cell culture supernatant using HWRGWV-WB resins constructed with different spacer arms (TREN, DADPA, mDADPA, and HMDA). Lanes: (1) Marker; (2) Load; (3) Flow-through; (4) Elution (pH 4.5); (5) Regeneration (pH 2.5).