Figure 1.

Effects of BRs treatment on cold stress tolerance in M. truncatula plants. Plants were pretreated with 1 μM BL or 1 μM BRZ or water (CK) to test BRs role. Both control (non-stressed) and cold stress (4 °C) plants’ leaf tissues were collected after 72 h for the detection of enzymes activities and MDA contents. The cold-treated plants photographed after 3 days (a), detection of ROS sites in leaves treated with BL (b), determination of MDA contents (c), and antioxidant enzymes activities (d–g). Bars represent mean and standard deviation of values obtained from three biological replicates. Significant differences (p < 0.05) are denoted by asterisks.