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Formation of a pre-initiation complex on the 30S ribosomal subunit (PDB 5LMV). IF1 (magenta), IF2 (blue), and IF3 (cyan) are present. The fMet-tRNAfMet (yellow) is bound in the P-site. Other colors are as in Figure 1. The fMet of the P-site fMet-tRNAfMet bound to IF2 is in the space-filling representation.
