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tRNA evolution. Homologous regions have the same color. Ac and T loop stem-loop-stems (17 nt) are red (initially ~CCGGGUUCAAAACCCGG). 5′-As (initially GCGGCGG) and homologous regions are yellow. 3′-As (initially CCGCCGC) and homologous regions are green. The D loop microhelix UAGCC repeat region (originally 17 nt) is magenta. Green spheres are Mg2+ ions.
