Figure 3.

Reduction of C. acnes colonization and inflammation in vivo by glycerol fermentation of S. epidermidis in PSF MTAM. The ears of Institute for Cancer Research (ICR) mice were injected intradermally with C. acnes (ATCC 6919) (10⁷ CFU) and topically applied with PSF MTAM loaded with (M+PSF+B+G) or without (M+PSF+G) S. epidermidis (ATCC 12228) (10⁵ CFU) in the presence of 2% glycerol in rich media. Three days after C. acnes injection, (a) The CFUs were enumerated by plating serial dilutions (1:10–1:10⁵) of the ear homogenate on an agar plate. (b) The levels of MIP-2 cytokines in the ears were measured by an enzyme-linked immunosorbent assay (ELISA) kit. Data are the means of three separate experiments using three mice per group. * p < 0.01; ** p < 0.001 (two-tailed t-tests).