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. 2018 Dec 31;24(1):135. doi: 10.3390/molecules24010135

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) HPLC-fluorescence chromatogram comparing f/2 blank (black line) and IRI-160AA (red line): 1 = DNS-OH, 2 = DNS-NH₃, 3 = DNS-glycine, and 4 =DNS-n-butylamine. Lower panels: Fragmentation patterns (MSMS spectra) of peaks 2 and 4 confirming the presence of DNS-NH₃ (B) and DNS-n-butylamine (C).