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HSCCC chromatogram of the crude sample from L. Japonicae Flos. Two-phase solvent system: ethyl acetate/n-butanol/methanol/water (5:1:1:5, v/v/v/v); stationary phase: upper phase; mobile phase: lower phase; flow-rate: 2.0 mL/min; revolution speed: 850 rpm; detection wavelength: 230 nm; separation temperature: 25 °C.
