Figure 2.
L-shape representations of M. mazei (Mm) tRNAPyl, PYLY1 (the naïve hybrid between Mm tRNAPyl and M. jannaschii tRNATyr), and two representative variants isolated from selections. The nucleotides in blue and black are derived from M. jannaschii tRNATyr and Mm tRNAPyl, respectively. The nucleotides in red show randomized positions. The identity elements of tRNAPyl and tRNATyr are underlined. A tertiary base pair in Mm tRNAPyl and presumed tertiary base pairs in the other tRNAs are indicated with grey boxes. Some nucleotides are numbered to highlight the difference between our numbering system and the standard one.