Table 1.
In vivo suppressor activities with 3-iodotyrosine and base sequences of M. jannaschii tRNATyr suppressors, M. mazei tRNAPyl, and the hybrid tRNAs between Mj tRNATyr and tRNAPyl molecules.
| Variant | Amber Suppression | Sequence 1 |
|---|---|---|
| MJR | Cm200 | CCGGCGGUAGUUCAGCCUGGUAGAACGGCGGACUCUAAAUCCGCAUGUCGCUGGUUCAAAUCCGGCCCGCCGGACCA |
| MJR1 | Cm200 | CCGGCGGUAGUUCAGCAGGGCAGAACGGCGGACUCUAAAUCCGCAUGGCGCUGGUUCAAAUCCGGCCCGCCGGACCA |
| Mm tRNAPyl | N. D. | GGAAACCUGAUCAUGUAGAUCGAAUGGACUCUAAAUCCGUUCAGCCGGGUUAGAUUCCCGGGGUUUCCGCCA |
| PYLY1 | Cm100 | CCGGCGGUGAUCAUGUAGAUCGAAUGGACUCUAAAUCCGUUCAGGCUGGUUAGAUUCCGGCCCGCCGGACCA |
| PYLY2 | Cm100 | CGAAACCUGAUCAUGUAGAUCGAAUGGACUCUAAAUCCGUUCAGCCGGGUUAGAUUCCCGGGGUUUCGACCA |
| PYLY1 (G1C72) |
< Cm25 | GCGGCGGUGAUCAUGUAGAUCGAAUGGACUCUAAAUCCGUUCAGGCUGGUUAGAUUCCGGCCCGCCGGACACCA |
| PYLY1 (U1A72) |
< Cm25 | UCGGCGGUGAUCAUGUAGAUCGAAUGGACUCUAAAUCCGUUCAGGCUGGUUAGAUUCCGGCCCGCCGGAAACCA |
| PYLY1 (G73) |
<Cm25 | CCGGCGGUGAUCAUGUAGAUCGAAUGGACUCUAAAUCCGUUCAGGCUGGUUAGAUUCCGGCCCGCCGGGCCA |
| PYLY1 (U73) |
<Cm25 | CCGGCGGUGAUCAUGUAGAUCGAAUGGACUCUAAAUCCGUUCAGGCUGGUUAGAUUCCGGCCCGCCGGUCCA |
| PYLY Dh |
Cm50 | CGGGGGUGGAUCGAAUAGAUCACACGGACUCUAAAUUCGUGCAGGCGGGUGAAACUCCCGUACUCCCGACCA |
| PYLY CMa |
Cm200 | CGGGGACGGUCCGGCGACCAGCGGGUCUCUAAAACCUGCCAGCGGGGUUCGACGCCCCGGUCUCUGACCA |
1 The D, T, and anticodon stems are underlined. The bases in blue and black letters are derived from M. jannaschii tRNATyr and tRNAPyl, respectively, when they coexist in a sequence. The base substitutions in MJR1 described in ref. 15 and the designed substitutions in the PYLY1 variants are shown in bold green letters.