Table 4.
In vivo suppressor activities with 3-iodotyrosine and base sequences in the cores of the PYLY1 and GGCT1 variants obtained from Selection 4.
| Variant | Amber Suppression | Sequence in the Core 1 |
|---|---|---|
| DT2-1 | Cm200 | 8UGAUCCUGGGGAUCG26…44CAGGCUGGUUCGAUUCCGGC65 |
| DT2-2 | Cm100 | 8UGAUCAAGGUGAUCG26…44CAGGCUGGUUCAAUUCCGGC65 |
| DT2-3 | Cm100 | 8UGAUCAUGGUGAUCG26…44CAGGCUGGUUCAACUCCGGC65 |
| DT2-4 | Cm50 | 8UGAUCAAGGAGAUCG26…44CAGGCUGGUUCGAUCCCGGC65 |
| DT3-1 | Cm200 | 8UGGCUCUGGGAGCCA26…44CUCGCUGGUUCGACUCCGGC65 |
| DT3-2 | Cm200 | 8UGGCUACGGUAGCCA26…44CUCGCUGGUUCGACCCCGGC65 |
| DT3-3 | Cm100 | 8UGGCUCUGGUAGCCA26…44CUCGCUGGUUCGAUUCCGGC65 |
| DT3-4 | Cm50 | 8UGGCUCGGGGAGCCA26…44CUCGCUGGUUCGAGUCCGGC65 |
| DT3-5 | Cm100 | 8UGGCUUAGGGAGCCA26…44CUCGCUGGUUCGAUUCCGGC65 |
1 The sequences from positions 8–26 and positions 44–65 are indicated. The complete sequences of the variants are shown in Table S1. The D and T stems are underlined. The bases in red and green letters show randomized positions and designed base substitutions, respectively. The bases in blue and black letters are derived from M. jannaschii tRNATyr and M. mazei tRNAPyl, respectively. The consensus G16, G17, U55, C56, and R57 are shown in bold letters.