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Topically administered KRFK peptide modulates effector phenotype in the draining lymph nodes of TSP-1-deficient mice. Flow cytometric analysis of phorbol 12-myristate 13-acetate (PMA)/ionomycin-stimulated lymph node cells harvested from peptide treated TSP-1 deficient mice were analyzed for intracellular IFN-γ, IL-17A, and Foxp3 staining in viable CD4+ cells. Total 10–20,000 CD4+ cells were analyzed per group. Control peptide: KQFK peptide.
