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Application of in-cell NMR in compound screening. Peptides that can disrupt FKBP12 and FRB interactions were screened using this approach. (a) 1H-15N-HSQC spectrum of FKBP12-FRB complex in which FKBP12 is 15N-labeled. (b) Peptide affects the spectrum of FKBP12-FRB complex. This figure was reprinted with permission from the reference [84]. Copyright (2009) American Chemical Society. For more experimental details, please refer to the original publication.
