. 2018 Dec 21;20(1):38. doi: 10.3390/ijms20010038

Table 3.

Blastocyst development and hatching rate of individual cultured bovine embryos after EV supplementation.

Treatment	System	No. of Presumed Zygotes	Cleavage Rate (%)	Blastocyst Yield (%)
Treatment	System	No. of Presumed Zygotes	Day 2	Day 7	Day 8	Hatching ^E
Control ^A	Group	225	82.2 ± 1.81 ^a	36.8 ± 1.20 ^a	49.7 ± 1.13 ^a	25.0 ± 0.92 ^a
Control ^B	Individual	226	87.5 ± 0.11 ^a	28.6 ± 0.06 ^b	40.4 ± 0.08 ^b	15.3 ± 0.87 ^b
Control+PBS ^C	Individual	232	89.3 ± 0.08 ^a	32.9 ± 0.14 ^a	40.7 ± 0.16 ^b	14.8 ± 1.03 ^b
EV supplemented ^D	Individual	306	83.9 ± 0.08 ^a	34.3 ± 0.09 ^a	46.7 ± 0.10 ^a	14.6 ± 1.09 ^b

Data are represented as mean ± SD. ^A Control = SOF+ITS+BSA medium used in group culture system (25 presumed zygotes/50 µL droplet); ^B Control = SOF+ITS+BSA medium used in individual culture system (1 presumed zygotes/20 µL droplet); ^C Control+PBS = SOF+ITS+BSA medium with PBS (1:400); ^D EV supplemented = SOF+ITS+BSA medium supplemented with EVs in PBS with an EV concentration of 0.228 µg/mL. ^E Hatching rates are shown as percentages of hatching or hatched blastocysts at 8 dpi compared with the total number of blastocysts. ^a,b Within each column, values that differ significantly are indicated by different superscripts (p < 0.05).