. 2018 Dec 21;20(1):38. doi: 10.3390/ijms20010038

Table 4.

Quality assessment of individual cultured bovine embryos after EV supplementation.

Treatment	System	No. of Embryos	Cell Number			ICM Ratio (%)	ACN	ACR (%)
Treatment	System	No. of Embryos	TCN	TE	ICM	ICM Ratio (%)	ACN	ACR (%)
Control	Group	36	120.8 ± 49.60 ^a	84.7 ± 40.93 ^a	36.1 ± 14.81 ^a	31.0 ± 10.50 ^a	7.14 ± 4.00 ^a	5.9 ± 2.39 ^a
Control^A	Individual	36	100.0 ± 26.72 ^b	67.4 ± 29.28 ^b	32.5 ± 6.31 ^a	35.5 ± 13.96 ^a	7.6 ± 4.28 ^a	8.3 ± 5.69 ^b
Control+PBS ^B	Individual	40	109.9 ± 30.63 ^a	77.7 ± 29.79 ^a	32.1 ± 6.28 ^a	30.9 ± 9.42 ^a	8.7 ± 4.41 ^a	8.2 ± 4.24 ^b
EV supplemented^C	Individual	38	109.5 ± 33.30 ^a	76.55 ± 32.66 ^a	33.0 ± 5.65 ^a	32.56 ± 10.32 ^a	3.5 ± 2.91 ^b	3.3 ± 2.90 ^a

Data are represented as mean ± SD. ^A Control = SOF+ITS+BSA medium used in group culture system (25 presumed zygotes/50µL droplet); ^B Control = SOF+ITS+BSA medium used in individual culture system (1 presumed zygotes/20µL droplet); ^C Control+PBS = SOF+ITS+BSA medium with PBS (1:400); ^D EV supplemented = SOF+ITS+BSA medium supplemented with EVs in PBS with an EV concentration of 0.228µg/mL. Total cell number (TCN) which includes the sum of both trophectoderm (TE) and inner cell mass cells (ICM), apoptotic cell number (ACN), ICM ratio and, apoptotic cell ratio (ACR) of blastocyst collected at 8 dpi were calculated after differential apoptotic staining. ^a–c Within each column, values that differ significantly are indicated by different superscripts (p < 0.05).