Table 3.
Box–Behnken Design (BBD) for the independent variables and corresponding response values.
| Runs | Time (X1)/min | Methanol Concentration (X2)/% | Temperature (X3)/°C | Solvent-to-Material Ratio (X4)/(mL/g) | Extraction Yield/mg.g−1 | Comprehensive Evaluation Value | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Trifolirhizin | Formononetin | Isoxanthohumol | Maackiain | Kurarinone | ||||||
| 1 | 40(0) | 70(0) | 70(0) | 25(0) | 2.117 | 0.172 | 0.432 | 0.574 | 2.428 | 1.040 |
| 2 | 40(0) | 60(−1) | 80(1) | 25(0) | 1.784 | 0.168 | 0.437 | 0.552 | 2.323 | 0.954 |
| 3 | 50(1) | 80(1) | 70(0) | 25(0) | 1.955 | 0.180 | 0.468 | 0.608 | 2.584 | 1.051 |
| 4 | 40(0) | 70(0) | 80(1) | 30(1) | 2.050 | 0.159 | 0.496 | 0.632 | 2.683 | 1.087 |
| 5 | 50(1) | 70(0) | 70(0) | 20(−1) | 1.901 | 0.136 | 0.457 | 0.585 | 2.556 | 1.015 |
| 6 | 40(0) | 60(−1) | 70(0) | 30(1) | 1.975 | 0.207 | 0.517 | 0.757 | 2.706 | 1.120 |
| 7 | 40(0) | 70(0) | 70(0) | 25(0) | 2.010 | 0.192 | 0.475 | 0.738 | 2.644 | 1.102 |
| 8 | 30(−1) | 70(0) | 70(0) | 20(−1) | 2.158 | 0.171 | 0.439 | 0.673 | 2.482 | 1.078 |
| 9 | 40(0) | 70(0) | 80(1) | 20(−1) | 2.277 | 0.179 | 0.487 | 0.709 | 2.709 | 1.155 |
| 10 | 40(0) | 70(0) | 60(−1) | 20(−1) | 2.195 | 0.200 | 0.522 | 0.666 | 2.948 | 1.183 |
| 11 | 30(−1) | 70(0) | 80(1) | 25(0) | 2.288 | 0.221 | 0.550 | 0.717 | 3.049 | 1.238 |
| 12 | 30(−1) | 60(−1) | 70(0) | 25(0) | 1.863 | 0.197 | 0.471 | 0.606 | 2.548 | 1.032 |
| 13 | 50(1) | 70(0) | 70(0) | 30(1) | 2.498 | 0.216 | 0.524 | 0.672 | 2.896 | 1.237 |
| 14 | 40(0) | 70(0) | 60(−1) | 30(1) | 1.980 | 0.214 | 0.493 | 0.640 | 2.639 | 1.085 |
| 15 | 40(0) | 70(0) | 70(0) | 25(0) | 2.036 | 0.198 | 0.500 | 0.624 | 2.748 | 1.107 |
| 16 | 40(0) | 70(0) | 70(0) | 25(0) | 2.364 | 0.163 | 0.495 | 0.641 | 2.718 | 1.154 |
| 17 | 40(0) | 60(−1) | 70(0) | 20(−1) | 1.809 | 0.114 | 0.416 | 0.457 | 2.414 | 0.936 |
| 18 | 30(−1) | 80(1) | 70(0) | 25(0) | 2.459 | 0.203 | 0.514 | 0.771 | 2.804 | 1.229 |
| 19 | 40(0) | 80(1) | 60(−1) | 25(0) | 2.489 | 0.208 | 0.522 | 0.782 | 2.926 | 1.260 |
| 20 | 50(1) | 70(0) | 60(−1) | 25(0) | 2.357 | 0.213 | 0.515 | 0.770 | 2.689 | 1.193 |
| 21 | 30(−1) | 70(0) | 60(−1) | 25(0) | 2.232 | 0.204 | 0.483 | 0.633 | 2.559 | 1.112 |
| 22 | 40(0) | 60(−1) | 60(−1) | 25(0) | 2.540 | 0.216 | 0.536 | 0.698 | 2.824 | 1.239 |
| 23 | 50(1) | 70(0) | 80(1) | 25(0) | 2.569 | 0.227 | 0.531 | 0.719 | 2.933 | 1.270 |
| 24 | 50(1) | 60(−1) | 70(0) | 25(0) | 2.212 | 0.204 | 0.488 | 0.627 | 2.539 | 1.104 |
| 25 | 30(−1) | 70(0) | 70(0) | 30(1) | 2.507 | 0.187 | 0.502 | 0.581 | 2.996 | 1.225 |
| 26 | 40(0) | 80(1) | 80(1) | 25(0) | 2.362 | 0.214 | 0.500 | 0.669 | 2.706 | 1.174 |
| 27 | 40(0) | 80(1) | 70(0) | 20(−1) | 2.266 | 0.151 | 0.443 | 0.637 | 2.441 | 1.077 |
| 28 | 40(0) | 70(0) | 70(0) | 25(0) | 2.654 | 0.214 | 0.638 | 0.801 | 1.579 | 1.076 |
| 29 | 40(0) | 80(1) | 70(0) | 30(1) | 2.490 | 0.177 | 0.476 | 0.695 | 2.597 | 1.170 |