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. 2019 Jan 1;20(1):131. doi: 10.3390/ijms20010131

Figure 1.

Figure 1

CCAR2 binds Hsp60 and survivin. Interaction between CCAR2 and survivin was examined in SH-SY5Y or HEK293 cells by co-immunoprecipitation with either an anti-CCAR2 or an anti-survivin antibody, followed by western blotting. (A) Interaction between CCAR2 and survivin in whole cell lysates from SH-SY5Y cells was examined. (B) Interaction between CCAR2, survivin, and Hsp60 in cytosolic and mitochondrial fractions isolated from HEK293 cells was examined. (C) SH-SY5Y cells were depleted of Hsp60 and the interaction between CCAR2 and survivin was examined. siU, universal siRNA; siH, Hsp60 siRNA.