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. 2019 Jan 5;20(1):176. doi: 10.3390/ijms20010176

Figure 3.

Figure 3

Evaluation of LGM2605 inhibition of NOD-like receptor protein 3 (NLRP3) activation in in vitro lung vascular networks (flow-adapted endothelial cells) exposed to low LET proton radiation. FAECs were exposed to 0 Gy or 1 Gy low LET (3–4 keV/µm) proton radiation and treated with 0 µM or 100 µM LGM2605 30 min following radiation exposure. Cells were harvested 24 h post radiation exposure and evaluated for NLRP3 inflammasome activation. Fluorescent images of FAECs exposed to LGM2605 (0 µM or 100 µM) 30 min following radiation exposure and harvested at 24 h radiation exposure and incubated with anti-NLRP3 antibody (R&D Systems, Minneapolis, MN, USA). Scale bar = 20 μm (A); graphed representation of the data imaged for each condition (B). Data are presented as mean ± SEM. # shown in figures indicate significant differences from FAECs exposed to 0 Gy low LET proton radiation and treated with 0 µM LGM2605 (#### p < 0.0001). Asterisks shown in figures indicate significant differences from FAECs exposed to 1 Gy low LET proton radiation and treated with 0 µM LGM2605 (*** p < 0.001).