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. 2019 Jan 5;20(1):176. doi: 10.3390/ijms20010176

Figure 7.

Figure 7

Evaluation of LGM2605 inhibition of ICAM-1 expression in in vitro lung vascular networks (flow-adapted endothelial cells) exposed to high LET proton radiation. FAECs were exposed to 0 Gy, 0.25 Gy, or 0.5 Gy high LET (8–10 keV/µm) proton radiation and treated with 0 µM or 100 µM LGM2605 30 min following radiation exposure. Cells were harvested 24 h post radiation exposure and evaluated for ICAM-1 expression. Fluorescent images of FAECs exposed to LGM2605 (0 µM or 100 µM) 30 min following radiation exposure and harvested at 24 h radiation exposure and incubated with anti-ICAM-1 antibody (ThermoFisher Scientific, Waltham, MA, USA). Scale bar = 20 μm (A); graphed representation of the data imaged for each condition (B). Data are presented as mean ± SEM. # shown in figures indicate significant differences from FAECs exposed to 0 Gy high LET proton radiation and treated with 0 µM LGM2605 (#### p < 0.0001). Asterisks shown in figures indicate significant differences from FAECs exposed to 0.25 Gy or 0.5 Gy high LET proton radiation and treated with 0 µM LGM2605, respectively (**** p < 0.0001).