Table 1.
Primary antibodies
| Antigen | Antibody | Species | Source | Concentration |
|---|---|---|---|---|
| Prph2/RRCT | RDS-CT | Rbt-PC | In house (15,16) | WB/IF 1:1000 |
| Prph2/RRCT | mAB 2B7, mAB 2E7 | Ms-MC | In house (49) | WB/IF 1:1000 |
| Prph2/RRCT | RDS-MPCT | Rbt-PC | Dr Andrew Goldberg (51,52) | IG 1:10 |
| Rom1 | ROM1-CT | Rbt-PC | In-house (15,16) | WB/IF 1:1000 |
| Rom1/RRCT | mAB 2H5 | Ms-MC | In-house (49,51) | WB/IF/IG 1:5 |
| Rhodopsin | Rhodopsin | Rbt-PC | Dr Steven Fliesler (35,40) | IG 1:10 |
| Rhodopsin | mAB 1D4 | Ms-MC | Dr Robert Molday (35,53) | IF 1:1000 |
| S-opsin | OPN1SW (N-20) | Gt-PC | Santa Cruz Biotechnology, cat# sc-14363 (6,49) | IF 1:500 |
| S-opsin | S-opsin | Rbt-PC | Dr Cheryl Craft (36,54) | IG 1:10 |
| M-opsin | Opsin 1 (Medium Wave) | Rbt-PC | Novus Biologicals cat# 110-74730 (55) | IF 1:1000 |
| Cone Arrestin | C-Arr | Rbt-PC | Dr Cheryl Craft (6,54) | IF 1:5000 |
| Syntaxin 3B | Syn3B | Rbt-PC | Synaptic Systems (cat# 110–032) (17,37) | IF 1:1000 |
| Acetylated Alpha-Tubulin | Ac-A-Tub, Clone 611B-1 | Ms-MC | Sigma (cat# T7451) (6) | IF 1:500 |
| Calreticulin | Calreticulin | Ckn-PC | Abcam (Ab #2908) (31,36) | IF 1:500 |
PC: polyclonal, MC: monoclonal, WB: western blot, IF: immunofluorescence, IG: immunogold. Epitope locations for Prph2/Rom1/RRCT antibodies are shown in Supplementary Material, Fig. 1.