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. 2018 Oct 12;28(3):487–500. doi: 10.1093/hmg/ddy363

Figure 6.

Figure 6

Expression of astrocyte-enriched proteins. Western blot analyses of astrocyte-enriched proteins in striatal protein homogenate from 13- to 14-month-old mice. (A) Western blots using Glt-1 and βactin as loading control (top panel), Kir4.1 and α-tubulin as loading control (middle panel) and αB-crystallin with βactin as loading control (bottom panel). Charts demonstrating no significant differences in the expression level of (B) Glt-1 [F (2,8) = 0.69, P = 0.54] or (C) Kir4.1, [F (2,8) = 3.4, P = 0.101] in the striatum of BACHD mice at this age. (D) Expression of αB-crystallin is changed in the group of samples [F (2,8) = 24.45, P = 0.0013]. It is decreased in BACHD mice (P = 0029) and normalized in BACHD/GFAP-CreERT2 mice (P = 0.7994) as compared to wild-type levels. BACHD/GFAP-CreERT2 levels are significantly increased as compared to BACHD levels. n = 3 BACHD, n = 3 BACHD/GFAP-CreERT2, n = 3 wild type. Data are mean ± SEM. **P < 0.004. One-way ANOVA and Tukey’s HSD post hoc test were used for analysis.