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. 2018 Dec 26;20(1):87. doi: 10.3390/ijms20010087

Figure 1.

Figure 1

Effect of luteinizing hormone (LH) on Krüppel-like factor 4 (Klf4), transcripts of apoptosis- and cell cycle-related genes in cultured preovulatory granulosa cells (GCs). Real-time RT-PCR analysis of transcripts of (A) Klf4; (B) Bax, and Bcl-2; and (C) Cyclins D1, D2, p21, and p27 in preovulatory GCs in response to LH (0 ng/mL, 100 ng/mL, and 200 ng/mL). All of the expression levels were normalized to 18S rRNA levels. Values were calculated as fold changes relative to untreated cells and are expressed as means ± standard deviations (SDs) of three separate experiments. LH, luteinizing hormone. * p < 0.05, vs. untreated cells; † p < 0.05, vs. cells treated with 100 ng/mL of LH.