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. 2018 Dec 26;20(1):87. doi: 10.3390/ijms20010087

Figure 2.

Figure 2

Regulation of apoptosis-related and cell cycle-related genes expression by Klf4 in preovulatory GCs. (A,C) Real-time RT-PCR analysis of Bax and Bcl-2 mRNA levels in GCs transfected with Klf4 expression vector (300 ng) and Klf4-specific siRNA (200 nM); (B,D) Real-time RT-PCR analysis of cyclins D1, D2, p21 and p27 mRNA levels in GCs transfected with Klf4 expression vector and Klf4-specific siRNA. 18S rRNA was used to normalize each reaction. Values are calculated as fold changes relative to empty vector (CT) or non-target siRNA (NT), and are expressed as the means ± SDs of three separate experiments. CT, cells transfected with empty vector; NT, cells transfected with non-target siRNA. * p < 0.05, ** p < 0.01 vs. CT or NT.