Table 1.
Primer sequences used for real-time PCR amplification.
| Gene | Primer Sequence (5′-3′) | Product Size (bp) | Accession Number |
|---|---|---|---|
| Klf4 | F: GAGAGGAACTCTCTCACATGAAGC | 185 | NM_053713.1 |
| R: AAGGATAAAGTCTAGGTCCAGGAGA | |||
| Bax | F: TGTTTGCTGATGGCAACTTC | 104 | NM_017059.1 |
| R: GATCAGCTCGGGCACTTTAG | |||
| Bcl-2 | F: GGGATGCCTTTGTGGAACTA | 138 | NM_016993.1 |
| R: CTCACTTGTGGCCCAGGTAT | |||
| Cyclin D1 | F: TCAAGTGTGACCCGGACTG | 213 | NM_171992.4 |
| R: CACTACTTGGTGACTCCCGC | |||
| Cyclin D2 | F: CGATGATCGCAACTGGAAGC | 232 | NM_022267.1 |
| R: TGGTCCGGATCTTCCACAGA | |||
| p21Cip1 | F: TGAGGGACCAGTACATGAGAACT | 192 | NM_031515.1 |
| R: GAGCCTGTTTCGTGTCTACTGTT | |||
| p27Kip1 | F: TCGCGGCTCCGAGACTT | 204 | NM_031762.3 |
| R: TCTCCAAGTCCCGGGTTAGT | |||
| 18S rRNA | F: CGCGGTTCTATTTTGTTGGT | 218 | M11188.1 |
| R: AGTCGGCATCGTTTATGGTC |
F, forward; R, reverse; p21Cip1, cyclin-dependent kinase-interaction protein 1; p27Kip1, cyclin-dependent kinase inhibitor 1B.