Table 2.
Calibration curves, LODs and LOQs data used for UPLC-MS/MS quantification of polyphenols and triterpenoids.
| Analyte | Ion Transition | Calibration Curve | R 2 | Linear Range (ng/mL) | LOD (ng/mL) | LOQ (ng/mL) |
|---|---|---|---|---|---|---|
| Quinic acid | 191 > 93 | y = 10.2 x + 0.06 | 0.9996 | 7843.1–784,313.6 | 13.1 | 78.4 |
| Arbutin | 317 > 161 | y = 19.9 x + 0.6 | 0.9999 | 3919.2–391,921.6 | 16.3 | 98.0 |
| (+)-Catechin | 289 > 289 | y = 270.9 x − 0.7 | 0.9997 | 73.5–14,703.9 | 2.5 | 7.4 |
| (−)-Epicatechin | 289 > 289 | y = 246.8 x + 1.7 | 0.9998 | 4.9–49,166.6 | 1.6 | 4.9 |
| Chlorogenic acid | 353 > 179 | y = 1.3 x − 1.1 | 0.9996 | 3923.1–196,156.8 | 65.4 | 392.3 |
| Cryptochlorogenic acid | 353 > 179 | y = 101.1 x + 30.0 | 0.9998 | 32.6–1302.6 | 10.9 | 32.6 |
| Neochlorogenic acid | 353 > 173 | y = 128.7 x + 4.1 | 0.9999 | 25.7–1029.8 | 8.6 | 25.7 |
| Isochlorogenic acid A | 515 > 353 | y = 82.1 x − 0.9 | 0.9998 | 66.9–33,427.8 | 5.6 | 33.4 |
| Isochlorogenic acid B | 515 > 353 | y = 287.7 x + 69.3 | 0.9998 | 10.5–524.3 | 3.5 | 10.5 |
| Isochlorogenic acid C | 515 > 353 | y = 267.2 x + 43.2 | 0.9997 | 17.3–690.3 | 2.9 | 17.3 |
| p-Coumaric acid | 163 > 163 | y = 1270.4 x + 90.3 | 0.9998 | 2.0–9817.6 | 0.2 | 2.0 |
| Caffeic acid | 179 > 179 | y = 165.7 x + 15.4 | 0.9998 | 9.8–98,352.8 | 1.6 | 9.8 |
| Rutin | 609 > 300 | y = 365.1 x + 2.1 | 0.9999 | 19.7–4912.7 | 1.6 | 9.8 |
| 609 > 609 | y = 478.3 x + 53.1 | 0.9998 | 4.9–9825.5 | 1.6 | 4.9 | |
| Kaempferol-3-O-rutinoside | 593 > 285 | y = 36.2 x + 0.7 | 0.9997 | 49.4–4941.2 | 19.8 | 49.4 |
| 593 > 593 | y = 90.2 x + 2.3 | 0.9997 | 19.8–4941.2 | 9.9 | 19.8 | |
| Isorhamnetin-3-O-rutinoside | 623 > 315 | y = 136.3 x + 5.3 | 0.9998 | 20.0–9982.3 | 5.0 | 20.0 |
| 623 > 623 | y = 342.7 x − 12.9 | 0.9999 | 10.0–9982.3 | 5.0 | 10.0 | |
| Luteoloside | 447 > 285 | y = 646.2 x + 95.2 | 0.9997 | 5.0–981.2 | 1.6 | 4.9 |
| 447 > 447 | y = 504.6 x − 11.0 | 0.9998 | 5.0–981.2 | 1.6 | 4.9 | |
| Hyperoside | 463 > 300 | y = 518.6 x + 20.7 | 0.9995 | 9.8–978.6 | 3.3 | 9.8 |
| Oleanic acid | 455 > 455 | y = 2546.2 x − 63.3 | 0.9999 | 9.8–982.2 | 0.8 | 2.0 |
| Ursolic acid | 455 > 455 | y = 2329.5 x + 44.9 | 0.9999 | 0.8–987.6 | 0.3 | 0.8 |
| Betulinic acid | 455 > 455 | y = 4256.9 x − 540.1 | 1.0000 | 0.8–489.7 | 0.3 | 0.8 |
| Pomolic acid | 471 > 471 | y = 1302.8 x − 22.8 | 0.9999 | 2.0–198.3 | 0.8 | 2.0 |
| Maslinic acid | 471 > 471 | y = 1255.5 x − 2.9 | 0.9999 | 2.0–493.1 | 0.8 | 2.0 |
| Corosolic acid | 471 > 471 | y = 1196.7 x − 8.6 | 0.9999 | 2.0–493.6 | 0.8 | 2.0 |