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. 2019 Jan 17;2(1):e201800131. doi: 10.26508/lsa.201800131

Figure S5. Rab1b or ARF5 depletion affects IRES activity.

Figure S5.

(A) The levels of Rab1b and ARF5 were determined by WB using anti-Rab1b or anti-ARF5 relative to siRNAcontrol-transfected cells. Tubulin was used as loading control. Rab1b- and ARF5-depleted cells were used to monitor relative IRES-dependent translation using monocistronic constructs. Each experiment was repeated three times. The effect on protein synthesis was calculated as % of luciferase activity/μg of protein relative to the control siRNA. Values represent the mean ± SD. An asterisk denotes statistically significant differences (P = 0.034) between cells treated with the siRNAcontrol or siARF5 RNA.