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. 2018 Dec 28;20(1):100. doi: 10.3390/ijms20010100

Figure 4.

Figure 4

Mutation in APIM in REV3L (F1241A) affects the mutation frequency and pattern. (a) Mutation frequency determined by the supF assay from overexpression of REV3L-YFP or REV3L F1241A-YFP in HEK 293 and POLη KO (HAP-1) cells. Cells expressing only UVB-irradiated pSP189 (supF reporter plasmid) represents the control. Four independent experiments are shown for each cell line. Students two-tailed paired t-test, * p < 0.05. (b,c) Mutation spectra (supF gene) isolated from cells overexpressing REV3L-YFP, REV3L F1241A-YFP, or only pSP189 (control) in HEK293 cells (b) and POLη KO cells (c) from four independent experiments. Left panel: Mutations at sites in the supF gene occurring with a frequency > 10% in either control (white bars), REV3L (light grey bars), or REV3L F1241A-expressing cells (dark grey bars). Right panel: Mutation spectra. The number in subscript indicates how many times the specific mutation was detected in the same transformation. Tandem and quadruple mutations are underlined.