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. 2018 Dec 28;20(1):100. doi: 10.3390/ijms20010100

Figure 6.

Figure 6

Treatment with APIM-peptide modulates the mutation spectra and reduces the mutation frequencies. (a,b) Mutation spectra (supF gene) from cells transfected with UVB irradiated pSP189 (supF reporter plasmid), with and without treatment with APIM-peptide (10 µM), isolated from (a) HEK293 and (b) REV3L ∆A1237 cells from four independent experiments. Left panel: Mutations at sites in the supF gene occurring with a frequency >10% in either control (white bars) or APIM-peptide treated cells (black bars). Right panel: Mutation spectra. The number in subscript indicates how many times the specific mutation was detected in the same transformation. Tandem mutations are underlined. (c) Mutation frequency in HEK293, REV3L ΔA1237, and XPA−/− cells after APIM-peptide treatment (black bars, 10 µM in HEK293 and REV3L ΔA1237, and 8 µM in XPA−/−) relative to untreated cells (white bars). Data from each cell line includes data from a minimum of three independent experiments. A significant reduction in mutation frequency after addition of APIM-peptide compared to control (untreated) was detected in all three cell lines (student two-tailed paired t-test, * p < 0.05).