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. 2018 Dec 28;20(1):105. doi: 10.3390/ijms20010105

Figure 5.

Figure 5

BAY11-7082 reduces ex vivo leukocyte adhesion induced by SAA in mice aorta. Mice were administered BAY11-7082 (5 mg/kg) or PBS (control) over a 14day period in parallel with administration of SAA (120 μg/kg; PBS as control) as described in the Methods Section. Aorta were subsequently isolated and subjected to ex vivo leukocyte adhesion studies using total labelled leukocytes. Arrows indicate leukocytes adhered to the aortic surface of mice treated with (A) SAA alone and (B) SAA + BAY11-7082. (C) Adherent leukocytes were quantified per field of view (FOV) by counting the number of dye-stained leukocytes that ceased motion during a 30 s recording at various time intervals under 160x magnification. Data represent mean ± SD. Independent aortic samples n = 4 SAA (time = 0; 2.5 min); n = 3 SAA (time = 5 min); n = 2 SAA (time = 7.5; 10 min); n = 6 SAA + BAY11-7082 (time = 0; 2.5; 5 min); n = 5 SAA + BAY11-7082 (time = 0 = 7.5; 10 min). * Different to SAA-only group ** p < 0.05 and *** p < 0.001. Data represent 3 technical and 3 biological replicates.