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. 2018 Dec 28;20(1):105. doi: 10.3390/ijms20010105

Figure 6.

Figure 6

ERK1/2 levels in aortae homogenates. Total phosphorylated ERK1/2 (p-ERK1/2) protein was analysed in homogenates prepared from isolated mouse aortae (refer to Methods section) using the commercial ERK1/2 (pT202/Y204) SimpleStep ELISA™ Kit (Abcam, Cambridge, MA, USA) (Catalogue number: ab176640) as per manufacturers guidelines. Absorbance readings were recorded at 450 nm using an Infinite® M200 PRO Plate reader (Tecan, Männedorf, Germany) and analysed using Microsoft Excel (2013, v7). Standard curves generated on the same plate were employed to express protein units in ug/mL. Homogenate samples tested were from control (n = 4), SAA (n = 2) and SAA + BAY11 (n = 4), each measured in duplicate. *** p < 0.0001 and ### p < 0.0001.