Fig. 6.

Schematic of secretion signal variants. The prototype secretion construct (SC0), and variations (SC1–SC11. Note that SC1 contributes to the ‘Mark IV’ improvement) are depicted along with the predicted size of the protein product (kDa). All secretion constructs harbour cutinase, along with combinations of the 3′UTR, 5′UTR, the 1–47 residue FliC secretion signal, or the truncated secretion signal (residues 26–47) with the native E. coli or S. typhimurium codon usage. Construction of these plasmids is outlined in Additional file 1: Fig. S5 and Table S2