Fig. 3.

The establishment of PCR-LFIA for CPV-2 detection. a PCR result determined by 1.5% agarose gel electrophoresis. Lane M, DL-2000 DNA marker. Lane 1–3, positive control CPV-2-N1. Lane 4–6, negative control. Lane 1′-6′, PCR products of positive and negative control purified by Magbeads PCR Purification Kit, respectively. b Corresponding fluorescence signals of test and control lines read by Nanoeasy 1700 for the same samples as in Fig. 3a