Fig. 5.

Sensitivity comparison of PCR-GE and PCR-LFIA for the detection of CPV-2. a Sensitivity of PCR-GE performed by 1.5% agarose gel electrophoresis. Lane M, DL-2000 DNA marker. Lane 2–10, 3 × 10⁸ copies/μL to 3 × 10⁰ copies/μL for standard DNA detected, respectively. Lane 11, negative control. b Corresponding signals of Test and Control lines for the same samples as in Fig. 5a. c Fluorescence signal values as a function of the copies of standard DNA detected. Each value was derived from three independent detections, and the error bars mean standard deviations