Table 1.
Effectiveness of PEG chain on PEGylated RBCs measured by agglutination (0 indicates no agglutination). Mal = Maleimide; CnCl = Cyanuric chloride. Reproduced with permission from John Wiley and Sons [75]
| Modifying PEG Reagenta | Agglutination with | |
|---|---|---|
| Anti-A | Anti-D | |
| None | 4+ | 3+ |
| Mal-Phe-PEG-5000 | 4+ | 0 |
| Mal-Phe-PEG-10000 | 2+ | 0 |
| Mal-Phe-PEG-20000 | 1+ | 1+ |
| Mal-Phe-PEG-5000 + 10,000 b | 4+ | 0 |
| Mal-Phe-PEG-5000 + 20,000 b | 1+ | 1+ |
| Mal-Phe-PEG-5000 + 10,000 + 20,000 b | 3+ | 0 |
| Mal-Phe-PEG-5000 and 20,000 c | 0 | 0 |
| CnCl-PEG-5000 | 4+ | 1+ |
aAll the incubations were carried out in the presence of IT
bThe PEG reagents of different chain lengths were present together in the reaction mixture
cThe RBCs were first PEGylated with the Mal-Phe-PEG-5000. After removing the excess reagents by washing, the PEGylated RBCs were reincubated with Mal-Phe-PEG-20000 in the presence of IT