Fig. 11.

Phylogeny of CSL transcription complexes. a Phylogenetic tree of the various animal clades from this study. Notch repressors Hairless, SHARP, and KyoT2 were not found outside the clade, where they have been originally identified. The Spen-like and Prickle-like family of proteins, however, is present in all the studied metazoan animals. b Phylogeny of Hairless evolution. Hairless can be traced back to Myriapoda that diverged from dipteran flies approximately 500 million years ago, i.e. after the Mandibulata-Chelicerata radiation. c Phylogeny of SHARP/MINT and KyoT2 evolution. SPEN-like proteins containing a RBPJ interacting domain (i.e. SHARP) are only found in vertebrates starting with arctic lampreys as example for Cyclostomata. Lampreys are considered living fossils that may not have changed morphologically the past 500 million years, but diverged about 600 million years back from mammals. The KyoT2 co-repressor, however, is only found in placental mammals. The CSL interacting motif in marsupial KyoT2 is quite diverged, presumably impeding the binding to CSL