FIGURE 3:

Predicted role of Drp1-lipid cooperativity during the constriction process. (a) The deformation response due to Drp1 and lipid generated curvatures from the 68% constricted state obtained in Figure 2b-4. (b) During the first phase, the curvature and stiffness of the Drp1 coat and the lipid localization is increased, causing the constriction to undergo a monotonic increase. (c) In the second phase, the coat is split and the two domains are moved apart. (d) Upon reaching a critical separation, the shape undergoes a second buckling instability, which yields a highly constricted state. The insets show the zoomed-in view of the constrictions. The red domain indicates the Drp1-coated region, and the adjacent blue domains show the regions of lipid localization.