FIGURE 3:

Comparison of the localization of junctional formins with TJ and AJ proteins. (A–D) 3×GFP-tagged Dia1 (A), Dia2 (B), Fhod1 (C), and Fhod3 (D) (green) were expressed together with TagBFP-ZO-1 (TJ marker; blue) and PLEKHA7-mCherry (AJ marker; red) in gastrula-stage X. laevis embryos and imaged by confocal microscopy. Top views (left) and averaged side views (right) of cell–cell junctions (50 pixels × 16 junctions) are shown. Scale bars: 10 µm (left); 2 µm (right). (E) Graphs indicate intensity profiles of formins (green solid line), ZO-1 (blue dotted line), and PLEKHA7 (red dotted line). Note that the intensity profiles of Dia1 and Dia2 are very similar to that of ZO-1 and that Fhod1 and Fhod3 have a peak at AJs as well as signal basal to the AJ. Error bars (vertical lines) indicate SD.