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. 2019 Jan 1;30(1):82–95. doi: 10.1091/mbc.E18-02-0133

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© 2019 Higashi et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — Ala-267 is necessary for junctional localization of Dia1. (A) Domain structure and mutants of Dia1. G, GTPase-binding domain; DID, Diaphanous inhibitory domain; DD, dimerization domain; FH, formin homology; DAD, Diaphanous autoregulatory domain. (B) Embryos expressing Dia1 WT or mutants (green) and mCherry-farnesyl (membrane probe; magenta) were observed. Note that the A267D/I842A mutant cannot localize at cell–cell junctions, but the I842A mutant can. Enlargements of cell–cell junctions (white boxes) are shown below. (C) Embryo expressing Dia1 A267D (green) and mCherry-farnesyl (magenta). Note that the cell expressing Dia1 A267D at high level (asterisk) is enlarged, probably due to cytokinesis failure. Scale bars: 10 µm (B); 40 µm (C).