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. 2019 Jan 1;30(1):82–95. doi: 10.1091/mbc.E18-02-0133

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© 2019 Higashi et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 5: — The DID-DD region is sufficient for junctional localization of Dia1. (A) Fragments of Dia1. The localization of CT was not directly assessed, because CT expression resulted in very large cell size (likely due to a cytokinesis defect) and compromised cell–cell junctions. (B) Embryos expressing mCherry-tagged Dia1 fragments (pseudocolored green) and GFP-farnesyl (membrane probe; pseudocolored magenta) were observed. Note that DID-DD is localized at cell–cell junctions, but DID-DD A267D is not. Both NT and CT cause abnormally large cell size. Scale bars: 10 µm.