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. 2019 Jan 1;30(1):30–41. doi: 10.1091/mbc.E18-08-0526

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FIGURE 2: — Homozygous FSS mutations Y583S, T1781, and R672C disrupt myofibril assembly and/or stability. Each panel contains transverse (top) and longitudinal (bottom) sections of a myofibril from homozygous transgenic lines. (A) Wild-type control (PwMhc2) late-stage pupa. Normal double-hexagonal packing of thick and thin filaments with oval shaped myofibril (top) as well as regular sarcomere structure (bottom) are observed. (B) Wild-type control (PwMhc2) 2-h-old adults. Normal structure is retained. (C) Wild-type control (PwMhc2) 2-d-old adult. Normal structure is retained. (D) Wild-type control (PwMhc2) 7-d-old adult. Normal structure is retained. (E) Y583S late-stage pupa resembles wild-type pupa, with normal double-hexagonal packing of thick and thin filaments and oval shaped myofibril (top) as well as regular sarcomere structure (bottom). (F) Y583S 2-h-old adult resembles wild-type 2-h-old adult, with normal double-hexagonal packing of thick and thin filaments and oval shaped myofibril (top) as well as regular sarcomere structure (bottom). (G) Y583S 2-d-old adult shows disrupted myofibril morphology with thick and thin filaments dispersing into neighboring myofibrils and gaps in the myofibrillar lattice. (H) Y583S 7-d-old adult shows continued disruption, with merging myofibrils. (I) T178I late-stage pupa shows poor myofibril morphology and disrupted hexagonal packing of myofilaments. Sarcomeres display gaps and fraying. (J) T178I 2-h-old adult shows further disruption of myofibril morphology, with myofilaments dispersed throughout the cell. (K) T178I 2-d-old adult shows disrupted morphology with Z-band material scattered within the myofibril. Sarcomeres lack defined M-lines and Z-bands. (L) R672C late-stage pupa shows irregularly shaped myofibrils, with some irregularities in sarcomere structure. (M) R672C 2-h-old adult shows variable sized myofibrils. Sarcomeres are poorly organized, with gaps and irregular borders. m, M-line; z, Z-band. Scale bars, 0.5 µm.