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. 2019 Jan 1;30(1):30–41. doi: 10.1091/mbc.E18-08-0526

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FIGURE 3: — FSS mutations Y583S, T1781, and R672C show dominant disruption of myofibril stability. Each panel contains transverse (top) and longitudinal (bottom) sections of a myofibril from heterozygous transgenic lines. (A) Wild-type control (PwMhc2/+) late-stage pupa. Normal double-hexagonal packing of thick and thin filaments with oval-shaped myofibril (top) as well as regular sarcomere structure (bottom) are observed. (B) Wild-type control (PwMhc2/+) 2-h-old adult. Normal structure is retained. (C) Wild-type control (PwMhc2/+) 2-d-old adult. Normal structure is retained. (D) Wild-type control (PwMhc2/+) 7-d-old adult. Normal structure is retained. (E) Y583S/+ late-stage pupa resembles control pupa, with normal double-hexagonal packing of thick and thin filaments and oval-shaped myofibril (top) as well as regular sarcomere structure (bottom). (F) Y583S/+ 2-h-old adult resembles control 2-h-old adult, with normal double-hexagonal packing of thick and thin filaments and oval-shaped myofibril (top) as well as regular sarcomere structure (bottom). (G) Y583S/+ 2-d-old adult shows fragmented myofibril morphology. (H) Y583S/+ 7-d-old adult shows continued disruption, with merging myofibrils as seen in homozygous Y583S 7-d-old adults (Figure 2H). (I) T178I/+ late-stage pupa resembles control pupa, with normal double-hexagonal packing of thick and thin filaments and oval-shaped myofibril (top) as well as regular sarcomere structure (bottom). (J) T178I/+ 2-h-old adult resembles control 2-h-old adult, with normal double-hexagonal packing of thick and thin filaments and oval-shaped myofibril (top) as well as regular sarcomere structure (bottom). (K) T178I/+ 2-d-old adult shows disrupted morphology with abnormal hexagonal packing of myofilaments within the myofibril. Sarcomeres are poorly organized. (L) R672C/+ late-stage pupa resembles control pupa, with normal double-hexagonal packing of thick and thin filaments and oval-shaped myofibril (top) as well as regular sarcomere structure (bottom). (M) R672C/+ 2-h-old adult shows myofibrillar degeneration with some loss of myofilament packing regularity. Sarcomeres are poorly organized. (N) R672C/+ 2-d-old adult. Disruption in myofibril morphology is more intense, with scattered Z-band material present. Sarcomeres show branching and fusing phenotypes with irregular M-lines/Z-bands. m, M-line; z, Z-band. Scale bars, 0.5 µm.