TABLE 1:
Transgenic lines and relative FSS myosin expression levels.
| Line name | Chromosome location | Protein accumulation ± SEM |
|---|---|---|
| PwMhc2 | X | 1.00 ± 0.04 |
| PwMhcY583S-5M | 3 | 0.98 ± 0.03 |
| PwMhcY583S-9M | 3 | 1.07 ± 0.08 |
| PwMhcY583S-11F | X | 0.95 ± 0.03 |
| PwMhcR672C-17 | 3 | 0.92 ± 0.06 |
| PwMhcR672C-19 | 3 | 0.94 ± 0.04 |
| PwMhcR672C-20 | 3 | 0.93 ± 0.06 |
| PwMhcT178I-3 | 3 | 0.93 ± 0.01 |
| PwMhcT178I-27 | 3 | 0.94 ± 0.01 |
Insertion sites and myosin expression levels for homozygous transgenic lines of T178I, Y583S, and R672C constructs in the Mhc10 (IFM myosin null) background. Protein was isolated from 2-d-old females. Myosin protein accumulation was determined relative to actin for each transgene by SDS–PAGE and densitometry. Two-three independent lines from for each FSS transgene are shown. Values are compared with PwMhc2 (wild type) control. Protein amounts are means ± SEM.