TABLE 2:

Flight ability of homozygous and heterozygous FSS mutants.

Line name	Age (d)	Number tested	Up (%)	Horizontal (%)	Down (%)	Not at all (%)	Flight index ± SEM
Homozygotes
PwMhc2	2	148	50.1	24.3	17.6	4.0	4.6 ± 0.02
PwMhcY583S-9M	2	117	0	0	0	100	0
PwMhcY583S-11F	2	120	0	0	0	100	0
PwMhcR672C-17	2	13	0	0	0	100	0
PwMhcR672C-20	2	15	0	0	0	100	0
PwMhcT178I-3	2	11	0	0	0	100	0
PwMhcT178I-27	2	23	0	0	0	100	0
Heterozygotes
PwMhc2/+	2	136	79.4	14.0	6.6	0	5.4 ± 0.02
PwMhcY583S-9M/+	2	134	27.6	29.1	23.1	20.2	3.28 ± 0.01
PwMhcY583S-11F/+	2	129	25.6	31.8	26.3	16.3	3.34 ± 0.01
PwMhcR672C-17/+	2	113	0	0	0	100	0
PwMhcR672C-20/+	2	125	0	0	0	100	0
PwMhcT178I-3/+	2	129	0	15.5	55.8	28.7	1.73 ± 0.01
PwMhcT178I-27/+	2	131	0	12.9	58.9	28.2	1.69 ± 0.01
PwMhc2/+	7	107	72	23	5	0	5.3 ± 0.01
PwMhcY583S-9M/+	7	144	19.4	26.4	29.2	25	2.81 ± 0.01
PwMhcY583S-11F/+	7	134	19.4	29.9	27.6	23.1	2.90 ± 0.02
PwMhcT178I-3/+	7	136	0	0	16.2	83.8	0.32 ± 0.01
PwMhcT178I-27/+	7	137	0	0	13.9	86.1	0.27 ± 0.01

Control (PwMhc2, PwMhc2/+) and FSS homozygotes and heterozygotes were assayed for the ability to fly up (U), horizontal (H), down (D), or not at all (N). Flight abilities are expressed as percentages of total number of flies tested. Two lines for each transgene are shown. Flight index was determined as 6U/T + 4H/T + 2D/T + 0N/T; T is the total number of flies tested in each cohort of 8–14 flies. Flight index mean and SEM were determined for the cohorts from each genotype. All mutant flight abilities are significantly different from the appropriate control and between 2 and 7 d of age, as determined by one-way ANOVA with Tukey's post-hoc test (p < 0.001).