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. 2019 Jan 1;30(1):56–68. doi: 10.1091/mbc.E18-05-0286

FIGURE 2:

FIGURE 2:

Fractones are visible as BM speckles in whole-mount V-SVZ immunostaining. (A) Whole-mount images of a mouse V-SVZ stained with an anti-panLM antibody. Left, image from the ventricle. Right, three-dimensional reconstruction of the left panel. See also Supplemental Movie S1. (B) Whole-mount V-SVZs were labeled with antibodies against individual BM proteins (red) together with anti-panLM or anti-LMγ1 antibodies (green). Each panel shows a merged image (top) and higher-magnification images (bottom) of the two channels in the boxed area. Asterisks, blood vessel BMs; arrowheads, speckled BMs. Scale bars, 10 μm.