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. 2018 Oct 12;21(1):92–97. doi: 10.4103/aja.aja_76_18

Figure 2.

Figure 2

Effects of nitrite and nitrate on 3-NT staining and DHE fluorescence in ipsilateral testis. Immunohistochemical staining of 3-NT in the left testes of Groups (a) A, (b) B, (c) C, and (d) G, respectively (scale bars = 50 μm). (e) The number of 3-NT-positive cells per seminiferous tubule in each group is shown. DHE fluorescence in the left testes of groups (f) A, (g) B, (h) C, and (i) G, respectively (scale bars = 50 μm). (j) The number of DHE-positive cells per seminiferous tubule in each group is shown. aP < 0.05 indicates significance compared to Group A, bP < 0.05 indicates significance compared to Group C. Group A: control; Group B: I/R injury; Group C: I/R + 0.12 nmol g−1 nitrite; Group G: I/R + 0.12 nmol g−1 nitrate. 3-NT: 3-nitrotyrosine; DHE: dihydroethidium; IR: ischemia/reperfusion.